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Top AI companies in Boston

Companies building AI products, foundation models, and applied ML platforms. Filtered to companies headquartered in Boston.

Built from a search like: “Top AI companies in Boston”

Ranked by current headcount across AI companies headquartered in Boston. Refreshed daily.

  1. 1
    Pacific Northwest National Laboratory· Richland, Washington, United States
    • Grant
    • 6.3k employees
    • Founded 1965
  2. 2
    Vertex Pharmaceuticals· Boston, Massachusetts, United States
    • post ipo secondary
    • 6.2k employees
    • Founded 1989
  3. 3
    Alexion Pharmaceuticals, Inc.· New Boston, Massachusetts, United States
    • Post-IPO equity
    • 5.1k employees
    • Founded 1992
  4. 4
    argenx· Boston, Massachusetts, United States
    • Post-IPO equity
    • 2.1k employees
    • Founded 2008
  5. 5
    Foundation Medicine· Boston, Massachusetts, United States
    • Series B
    • 1.8k employees
    • Founded 2010
  6. 6
    Cobb· Siloam Springs, Arkansas, United States
    • 1.3k employees
    • Founded 1916
  7. 7
    Care Access· Boston, Massachusetts, United States
    • Grant
    • 838 employees
    • Founded 2015
  8. 8
    Ginkgo Bioworks· Boston, Massachusetts, United States
    • 644 employees
    • Founded 2009
  9. 9
    Instem· Boston, Massachusetts, United States
    • Grant
    • 517 employees
    • Founded 1969
  10. 10
    Velsera· Boston, Massachusetts, United States
    • 436 employees
    • Founded 2023
  11. 11
    Rhythm Pharmaceuticals Inc.· Boston, Massachusetts, United States
    • Post-IPO equity
    • 435 employees
    • Founded 2008
  12. 12
    Nucleate· Boston, Massachusetts, United States
    • 428 employees
    • Founded 2019
  13. 13
    Wyss Institute at Harvard University· Boston, Massachusetts, United States
    • 372 employees
  14. 14
    CRISPR Therapeutics· Boston, Massachusetts, United States
    • Post-IPO debt
    • 366 employees
    • Founded 2013
  15. 15
    Ironwood Pharmaceuticals· Boston, Massachusetts, United States
    • Post-IPO debt
    • 261 employees
    • Founded 1998
  16. 16
    The Martinos Center for Biomedical Imaging· Boston, Massachusetts, United States
    • 254 employees
    • Founded 2000
  17. 17
    Fortis Life Sciences· Boston, Massachusetts, United States
    • 222 employees
    • Founded 2020
  18. 18
    Monte Rosa Therapeutics· Boston, Massachusetts, United States
    • Post-IPO equity
    • 208 employees
    • Founded 2018
  19. 19
    Lindus Health· Boston, Massachusetts, United States
    • Grant
    • 202 employees
    • Founded 2021
  20. 20
    AVEO Oncology· Boston, Massachusetts, United States
    • Post-IPO equity
    • 201 employees
    • Founded 2001
  21. 21
    Entrada Therapeutics· Boston, Massachusetts, United States
    • Post-IPO equity
    • 199 employees
    • Founded 2016
  22. 22
    Roslin Institute· Bilston, Scotland, United Kingdom
    • 196 employees
  23. 23
    Praxis Precision Medicines, Inc.· Boston, Massachusetts, United States
    • Post-IPO equity
    • 192 employees
    • Founded 2015
  24. 24
    Lux Research· Boston, Massachusetts, United States
    • Private equity
    • 185 employees
    • Founded 2004
  25. 25
    Akili· Boston, Massachusetts, United States
    • Series D
    • 175 employees
    • Founded 2015

Find engineers at AI companies in Boston

Boston has long been a hub for AI, and the list above ranks the largest AI companies headquartered there by current headcount, drawn from public LinkedIn and Crunchbase records. With 301 matching companies in our index, this is a good map of where the AI talent in Bostonlives — useful whether you’re recruiting, partnering, or sizing a market.

The biggest AI companies in Boston tend to be the gravitational centres for specialised talent — senior engineers, ML researchers, applied scientists, designers — while the smaller ones compete on speed, equity, and proximity to founders.

Refolk converts this list into a live search. Ask for “AI engineers in Bostonhiring senior staff” or “founders of AI companies in Bostonwho left in the last 12 months” and Refolk returns a ranked shortlist with the evidence that earned each person a spot.

How this list is built

Refolk pulled AI companies headquartered in Boston from public LinkedIn and Crunchbase records, ranked them by current employee count, and kept those with at least 10 employees. The list refreshes once a day.

Last refreshed: Thu, 07 May 2026 06:34:07 GMT

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